-
Product Name
Anti-V5 antibody
- Documents
-
Description
Mouse Monoclonal to V5
-
Tested applications
WB, IP
-
Species reactivity
Recognize N-terminal and C-terminal V5 Tag in fusion proteins
-
Isotype
Mouse IgG1
-
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
-
Clonality
Monoclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 1-5 μg/ml
IP: 1-4 μg/mg of lysate
-
Validations
Western blot (WB) 14828
Immunoprecipitation(IP) 14830
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"