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Product Name
Anti-ULK3 Rabbit antibody
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Description
ULK3 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human ULK3
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000-1/5000
ICC/IF: 1/20-1/50
FC: 1/20
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Validations
Western blot detection of ULK3 in K562,Hela cell lysates using ULK3 Rabbit pAb(1:1000 diluted).Predicted band size:53kDa.Observed band size:53kDa.
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Background
Swiss-Prot Acc.Q6PHR2.Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"