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Product Name
Anti-Ulk2 antibody
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Description
Mouse monoclonal antibody to Ulk2
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Tested applications
WB, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
Unc51. antibody; Unc51.2 antibody; AU015340 antibody; mKIAA0623 antibody; A830085I22Rik antibody
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Isotype
Mouse IgG1
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Monoclonal
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Formulation
Liquid, 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:2,000
FC: 1:100-1:200
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Validations
Fig1: Western blot analysis of ULK2 on human ULK2 recombinant protein using anti-ULK2 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of ULK2 on HEK293 (1) and ULK2-hIgGFc transfected HEK293 (2) cell lysate using anti-ULK2 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of ULK2 on different cell lysate using anti-ULK2 antibody at 1/1,000 dilution.; Positive control:; Lane 1: NIH/3T3; Lane 2: HepG2; Lane 3: SK-Hep-1; Lane 4: SK-OV-3; Lane 5: C6; Lane 6: PC-12; Lane 7: MCF-7
Fig4: Flow cytometric analysis of Hela cells with ULK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
- Background
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References
- Puto LA et al. Transcriptional Repressor DAXX Promotes Prostate Cancer Tumorigenicity via Suppression of Autophagy. J Biol Chem 290:15406-20 (2015).
- Konno H et al. Cyclic Dinucleotides Trigger ULK1 (ATG1) Phosphorylation of STING to Prevent Sustained Innate Immune Signaling. Cell 155:688-98 (2013).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"