Anti-UBE2W antibody

Anti-UBE2W antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

Mouse monoclonal to UBE2W

WB

Human UBE2W

ubiquitin-conjugating enzyme 16 antibody; ubiquitin-conjugating enzyme E2 W antibody; ubiquitin-protein ligase W antibody; hUBC-16 antibody; probable ubiquitin-conjugating enzyme E2 W antibody; UBC16 antibody; UBC-16 antibody; ubiquitin carrier protein W antibody; UBC16 antibody; UBC-16 antibody; 6130401J04Rik antibody

Mouse IgG

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human UBE2W (rh UBE2W; Q96B02-12; Met1-Cys151). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Monoclonal

0.2 μm filtered solution in PBS with 5% trehalose

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Ubiquitin-conjugating enzymes, also known as UBE2W, E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step of protein ubiquitination. The modification of protein with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2W is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It also catalyzes monoubiquitination and "Lys-11"-linked polyubiquitination. UBE2W is also considered to regulate FANCD2 monoubiquitination. UBE2W exhibits ubiquitin conjugating enzyme activity and catalyzes the monoubiquitination of PHD domain of Fanconi anemia complementation group L (FANCL). Over-expression of UBE2W in cells promotes the monoubiquitination of FANCD2 and down-regulated UBE2W markedly reduces the UV irradiation-induced but not MMC-induced FANCD2 monoubiquitination.

• Zhang Y, et al. (2008) Generation of mouse UBE2W antibody and analysis of UBE2W expression in mouse tissues. Sheng Wu Gong Cheng Xue Bao. 24(4): 547-52.
• Scaglione KM, et al. (2011) Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP. Mol Cell. 43(4): 599-612.
• Zhang Y, et al. (2011) UBE2W interacts with FANCL and regulates the monoubiquitination of Fanconi anemia protein FANCD2. Mol Cells. 31(2): 113-22.