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Product Name
Anti-UBE2D1 antibody
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Description
Rabbit polyclonal to UBE2D1
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Tested applications
ELISA, WB
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Species reactivity
Human UBE2D1 / UBCH5
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Alternative names
E2(17)KB1 antibody; MGC28550 antibody; SFT antibody; UBCH5 antibody; UBCH5 antibody; UBE2D1 antibody; Ube2d1 antibody; UBC4/5 antibody; SFT antibody; UBCH5 antibody; UBC4/5 antibody; UBCH5A antibody; E2(17)KB1 antibody; UBCH5 antibody; UBCH5A antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human UBE2D1 / UBCH5 (rh UBE2D1 / UBCH5; P51668; Ala2-Met147). UBE2D1 / UBCH5 specific IgG was purified by Human UBE2D1 / UBCH5 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human UBE2D1 / UBCH5. The detection limit for Human UBE2D1 / UBCH5 is approximately 0.00975 ng/well.
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Validations
UBE2D1 / UBCH5 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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References
- Zhang L, et al. (2011) The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor. Genes Dev. 25(12): 1262-74.
- Tokumoto M, et al. (2011) Cadmium toxicity is caused by accumulation of p53 through the down-regulation of Ube2d family genes in vitro and in vivo. J Toxicol Sci. 36(2): 191-200.
- Ohbayashi N, et al. (2008) Physical and functional interactions between ZIP kinase and UbcH5. Biochem Biophys Res Commun. 372(4): 708-12.
- Gehrke SG, et al. (2003) UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis. Blood. 101(8): 3288-93.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"