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Product Name
Anti-TWF1/Twinfilin-1 (5F10) Mouse antibody
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Description
TWF1/Twinfilin-1 (5F10) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human TWF1 (AA: 335-384 ) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/100
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TWF1 mouse mAb with DAB staining.
Immunofluorescence analysis of HeLa cells using TWF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TWF1 mouse mAb with DAB staining.
Flow cytometric analysis of HeLa cells using TWF1 mouse mAb (green) and negative control (red).
Western blot analysis using TWF1 mAb against HEK293 (1) and TWF1 (AA: 335-384)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot detection of Twinfilin-1 in MCF7,C6,3T3 and Hela cell lysates using Twinfilin-1 mouse mAb (1:5000 diluted).Predicted band size:40KDa.Observed band size:40KDa.
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Background
Swiss-Prot Acc.Q12792.This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.
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