Anti-TUBA8 antibody

Cat.#: 175060

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Product Information

  • Product Name
    Anti-TUBA8 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to TUBA8
  • Tested applications
    WB, ICC, IHC-P, FC
  • Species reactivity
    Human, Rat
  • Alternative names
    CDCBM8 antibody; TUBAL2 antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    ICC: 1:100-1:500

    IHC-P: 1:100-1:500

    FC: 1:100-1:200

  • Validations

    Fig1: Western blot analysis of TUBA8 on human TUBA8 recombinant protein using anti-TUBA8 antibody at 1/1,000 dilution.

    Fig1: Western blot analysis of TUBA8 on human TUBA8 recombinant protein using anti-TUBA8 antibody at 1/1,000 dilution.

    Fig2: Western blot analysis of TUBA8 on at heart tissue lysate using anti-TUBA8 antibody at 1/1,000 dilution.

    Fig2: Western blot analysis of TUBA8 on at heart tissue lysate using anti-TUBA8 antibody at 1/1,000 dilution.

    Fig3: ICC staining TUBA8 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining TUBA8 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues using anti-TUBA8 antibody. Counter stained with hematoxylin.

    Fig4: Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues using anti-TUBA8 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using anti-TUBA8 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using anti-TUBA8 antibody. Counter stained with hematoxylin.

    Fig6: Flow cytometric analysis of NIH/3T3 cells with TUBA8 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

    Fig6: Flow cytometric analysis of NIH/3T3 cells with TUBA8 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

  • Background
  • References
    • Ehnert S et al. TGF-β1 impairs mechanosensation of human osteoblasts via HDAC6-mediated shortening and distortion of primary cilia. Journal of molecular medicine (Berlin, Germany). 95: 653-663 (2017).
    • Noack K et al. Analysis of the interplay between all-trans retinoic acid and histone deacetylase inhibitors in leukemic cells. Archives of toxicology. 91: 2191-2208 (2017).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"