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Product Name
Anti-TRIM25/EFP Rabbit antibody
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Description
TRIM25/EFP Rabbit monoclonal antibody
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Tested applications
WB, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
EFP; Z147; RNF147; ZNF147 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human TRIM25
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Clonality
Monoclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/5000
ICC/IF: 1/50-1/200
FC: 1/20
IP: 1/20
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Validations
Western blot detection of TRIM25/EFP in K562,Rat Brain,C6,Hela cell lysates using TRIM25/EFP Rabbit mAb(1:1000 diluted).Predicted band size:71kDa.Observed band size:71kDa.
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Background
Swiss-Prot Acc.Q14258.Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"