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Anti-TREM2 antibody

Cat.#: 175794

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-TREM2 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to TREM2
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Rat
  • Alternative names
    PLOSL2 antibody; TREM-2 antibody; Trem2a antibody; Trem2b antibody; Trem2c antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within human trem2 aa 180-230.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500

    FC: 1:50-1:100

  • Validations

    Fig1:; Western blot analysis of Trem2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: THP-1 cell lysate; Lane 2: Rat bone marrow tissue lysate

    Fig1:; Western blot analysis of Trem2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: THP-1 cell lysate; Lane 2: Rat bone marrow tissue lysate

    Fig2:; Flow cytometric analysis of Trem2 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Fig2:; Flow cytometric analysis of Trem2 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Background
    Q9NZC2
  • References
    • Bouchon A. et. al. Inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes.J. Immunol. 164:4991-4995 (2000).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"