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Product Name
Anti-TGM2 / Transglutaminase 2 antibody
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Description
Rabbit polyclonal to TGM2 / Transglutaminase 2
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Tested applications
ELISA, WB, IP
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Species reactivity
Human TGM2 / TGC / Transglutaminase-2
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Alternative names
TG2 antibody; TGC antibody; GNAH antibody; HEL-S-45 antibody; G-ALPHA-h antibody; TG2 antibody; tTG antibody; G[a]h antibody; tTGas antibody; TGase2 antibody; GNAH antibody; RP5-1054A22.2 antibody; C polypeptide antibody; C polypeptide antibody; G[a]h antibody; G-ALPHA-h antibody; OTTMUSP00000016945 antibody; RP23-396G1.2 antibody; TG C antibody; TG(C) antibody; TG(C) antibody; TG2 antibody; TG2 antibody; TGase C antibody; TGase C antibody; TGase2 antibody; TGase-2 antibody; TGase-2 antibody; TGase H antibody; TGC antibody; TGC antibody; TGM2 antibody; TGM2 antibody; Transglutaminase 2 antibody; Transglutaminase 2 antibody; tTG antibody; tTGas antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human TGM2 / Transglutaminase-2 (rh TGM2; NP_004604.2; Met 1-Ala 687). TGM2 specific IgG was purified by human TGM2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human TGM2. The detection limit for Human TGM2 is approximately 0.00975 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
TGM2 / Transglutaminase 2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
TGM2 / Transglutaminase 2 Antibody, Rabbit PAb, Antigen Affinity Purified
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Background
Protein-glutamine gamma-glutamyltransferase 2, also known as Tissue transglutaminase, Transglutaminase C, Transglutaminase-2, and TGM2, is a member of the transglutaminase superfamily. TGM2 plays a role in cell growth and survival through the anti-apoptosis signaling pathway. It is a calcium-dependent acyltransferase which also undergoes a GTP-binding/GTPase cycle even though it lacks any obvious sequence similarity with canonical GTP-binding (G) proteins. TGM2 is a multi-functional protein which catalyzes transamidation reactions or acts as a G-protein in intracellular signalling. As an enzyme which is responsible for the majority of transglutaminase (TG) activity in the brain, TGM2 is likely to play a modulatory role in nervous system development and has regulatory effect on neuronal cell death as well. Most importantly, numerous studies have presented data demonstrating that dysregulation of TGM2 may contribute to the pathogenesis of many neurodegenerative disorders, including Huntington's disease, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis as well as nervous system injuries.
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References
- Ruan Q, et al. (2007) Transglutaminase 2 in neurodegenerative disorders. Front Biosci. 12: 891-904.
- Ai L, et al. (2008) The transglutaminase 2 gene (TGM2), a potential molecular marker for chemotherapeutic drug sensitivity, is epigenetically silenced in breast cancer. Carcinogenesis. 29(3): 510-8.
- Filiano AJ, et al. (2010) Transglutaminase 2 protects against ischemic stroke. Neurobiol Dis. 39(3): 334-43.
- Park D, et al. (2010) Transglutaminase 2: a multi-functional protein in multiple subcellular compartments. Amino Acids. 39(3): 619-31.
- Miyoshi N, et al. (2010) TGM2 is a novel marker for prognosis and therapeutic target in colorectal cancer. Ann Surg Oncol. 17(4): 967-72.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"