Anti-TBCC antibody

Cat.#: 175049

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Product Information

  • Product Name
    Anti-TBCC antibody
  • Documents
  • Description
    Mouse monoclonal antibody to TBCC
  • Tested applications
    WB, IHC-P, ICC
  • Species reactivity
    Human
  • Alternative names
    CFC antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    ICC: 1:50-1:200

    IHC-P: 1:50-1:200

  • Validations

    Fig1: Western blot analysis of TBCC on human TBCC recombinant protein using anti-TBCC antibody at 1/1,000 dilution.

    Fig1: Western blot analysis of TBCC on human TBCC recombinant protein using anti-TBCC antibody at 1/1,000 dilution.

    Fig2: Western blot analysis of TBCC on HEK293 (1) and TBCC-hIgGFc transfected HEK293 (2) cell lysate using anti-TBCC antibody at 1/1,000 dilution.

    Fig2: Western blot analysis of TBCC on HEK293 (1) and TBCC-hIgGFc transfected HEK293 (2) cell lysate using anti-TBCC antibody at 1/1,000 dilution.

    Fig3: ICC staining TBCC (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining TBCC (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-TBCC antibody. Counter stained with hematoxylin.

    Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-TBCC antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-TBCC antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-TBCC antibody. Counter stained with hematoxylin.

  • Background
  • References
    • Hage-Sleiman R et al. Tubulin binding cofactor C (TBCC) suppresses tumor growth and enhances chemosensitivity in human breast cancer cells. BMC Cancer 10:135 (2010).
    • Si F et al. Study on H2O2/TAED and H2O2/TBCC bleaching mechanism related to hydroxyl radical with a fluorescent probe. Carbohydr Polym 103:581-6 (2014).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"