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Product Name
Anti-SUB1 / PC4 antibody
- Documents
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Description
Mouse monoclonal to SUB1 / PC4
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Tested applications
WB
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Species reactivity
Human SUB1 / PC4
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Alternative names
P14 antibody; P14 antibody; P15 antibody; activated RNA polymerase II transcription cofactor 4 antibody; activated RNA polymerase II transcriptional coactivator p15 antibody; activated RNA polymerase II transcriptional coactivator p15 antibody; AI842364 antibody; P15 antibody; P9 antibody; PC4 antibody; Pc4 antibody; positive cofactor 4 antibody; positive cofactor 4 antibody; RNA polymerase II transcriptional coactivator antibody; Rpo2tc1 antibody; single-stranded DNA-binding protein p9 antibody; Sub1 antibody; SUB1 antibody; SUB1 homolog (S. cerevisiae) antibody; SUB1 homolog (S. cerevisiae) antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SUB1 / PC4 (rh SUB1 / PC4; P53999; Met1-Leu127). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% treha
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
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Validations
SUB1 / PC4 Antibody, Mouse MAb, Western blot
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Background
SUB1 belongs to the transcriptional coactivator PC4 family. It is a general coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SUB1 binds single-stranded DNA. Single-stranded DNA-binding proteins play many roles in nucleic acid metabolism, but their importance during transcription remains unclear. SUB1 exhibits strong genetic interactions with factors necessary for promoter melting. It localizes near the transcription bubble in vitro and binds to promoters in vivo dependent upon preinitiation complexes assembly. SUB1 interacts with the nontemplate strand of RNApII complexes during initiation. It may also be involved in stabilizing the multiprotein transcription complex.
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References
- Knaus R, et al. (1996) Yeast SUB1 is a suppressor of TFIIB mutations and has homology to the human co-activator PC4. EMBO J. 15(8):1933-40.
- Ge H, et al. (1994) Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes. Cell. 78(3):513-23.
- Kaiser K, et al. (1994) A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators. Cell. 78(3):525-34.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"