-
Product Name
Anti-STX8 / Syntaxin 8 antibody
- Documents
-
Description
Rabbit polyclonal to STX8 / Syntaxin 8
-
Tested applications
WB, IP
-
Species reactivity
Human STX8 / Syntaxin 8
-
Alternative names
syntaxin 8 antibody; STX8 antibody; CARB antibody; CIP-1-associated regulator of cyclin B antibody; syntaxin 8 antibody; Stx8 antibody; RP23-25D18.1 antibody; 0610007H08Rik antibody; 1110002H11Rik antibody; 4930571E13Rik antibody; CARB antibody; syntaxin-like protein 3I35 antibody; CARB antibody; CARB antibody; 0610007H08Rik antibody; 1110002H11Rik antibody; 4930571E13Rik antibody
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human STX8 / Syntaxin 8, and purified by antigen affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 0.5-5 μg/ml
IP: 1-4 μg/mg of lysate
-
Validations
STX8 / Syntaxin 8 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
STX8 / Syntaxin 8 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
-
Background
STX8, also known as syntaxin 8, directly interacts with HECTd3. STX8 forms the SNARE complex with syntaxin 7, vti1b and endobrevin. STX8 belongs to the syntaxin family. Members of this family are key molecules implicated in diverse vesicle docking and membrane fusion events. STX8 physically interacts with cystic fibrosis transmembrane conductance regulator (CFTR): recombinant syntaxin 8 binds CFTR in vitro and both proteins co-immunoprecipitate in HT29 cells. Syntaxin 8 regulates CFTR-mediated currents in chinese hamster ovary (CHO) cells stably expressing CFTR and syntaxin 8. STX8 contributes to the regulation of CFTR trafficking and chloride channel activity by the SNARE machinery.
-
References
- Steegmaier M. et al., 1999, J Biol Chem. 273 (51): 34171-9.
- Thoreau V. et al., 1999, Biochem Biophys Res Commun. 257 (2): 577-83.
- Zhang L. et al., 2009, Cell Mol Neurobiol. 29 (1): 115-21.
- Bilan F. et al., 2004, J Cell Sci. 117 (10): 1923-35.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"