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Product Name
Anti-SPARCL1 / SPARC-like 1 antibody
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Description
Rabbit monoclonal to SPARCL1 / SPARC-like 1
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Tested applications
IF, ICC/IF
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Species reactivity
Human SPARCL1 / SPARC-like 1
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Alternative names
Ecm2 antibody; extracellular matrix protein 2 antibody; hevin antibody; hevin antibody; hevin) antibody; hevin) antibody; mast9 antibody; SC1 antibody; mast9 antibody; PIG33 antibody; proliferation-inducing protein 33 antibody; Sc1 antibody; Sparcl1 antibody; SPARCL1 antibody; SPARC-like 1 (mast9 antibody; SPARC-like 1 (mast9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human SPARCL1 / SPARC-like 1 (rh SPARCL1 / SPARC-like 1; NP_004675.3; Met1-Phe664).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ICC/IF: 10-25 μg/mL
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Validations
SPARCL1 / SPARC-like 1 Antibody, Rabbit MAb, Immunofluorescence
Immunofluorescence staining of Human SPARCL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SPARCL1 monoclonal antibody (15 µg/ml). Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoplasm.
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Background
SPARC-like protein 1 (SPARCL1; also known as SC1, high endothelial venule protein, or hevin) is an extracellular matrix-associated, secreted glycoprotein belonging to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins. It contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation. SPARCL1 is expressed during embryogenesis and tissue remodeling and is especially prominent in brain and vasculature. Its down-regulation in a number of cancers and the possibility of its functional compensation by SPARC has led to recent interest in hevin as a tumor suppressor and regulator of angiogenesis. SPARCL1 has antiadhesive properties, and loss of SPARCL1 expression is associated with increased proliferative activity and cell cycle progression. It is suggested that it may influence multiple cellular processes during distinct stages of brain development and function. In addition, SPARCL1 can influence the function of astroglial cells in the developing and mature central nervous system (CNS).
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References
- Sullivan MM, et al. (2004) Hevin/SC1, a matricellular glycoprotein and potential tumor-suppressor of the SPARC/BM-40/Osteonectin family. Int J Biochem Cell Biol. 36(6): 991-6.
- Esposito I, et al. (2007) Tumor-suppressor function of SPARC-like protein 1/Hevin in pancreatic cancer. Neoplasia. 9(1): 8-17.
- Weimer JM, et al. (2008) A BAC transgenic mouse model to analyze the function of astroglial SPARCL1 (SC1) in the central nervous system. Glia. 56(9): 935-41.
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