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Product Name
Anti-SP7 antibody
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Description
Rabbit polyclonal antibody to SP7
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit
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Alternative names
OSX antibody; OI11 antibody; OI12 antibody; osterix antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human osterix 331-431/431
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
IHC-P:1:400-800
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Validations
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse embryos); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Osterix) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:; Embryo(Mouse) lysate at 30ug;; Embryo(Mouse) lysate at 30ug;; Primary: Anti-Osterix at 1:200 dilution;; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;; Predicted band size : 45kD; Observed band size : 45kD
Fig3: Sample:Spinal cord (Mouse) Lysate at 40 ug; Primary: Anti-Osterix at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 45kD; Observed band size: 50kD
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"