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Product Name
Anti-SOX9 Rabbit antibody
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Description
SOX9 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human SOX9
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/2000
IHC: 1/100-1/500
ICC/IF: 1/50-1/200
IP: 1/50
FC: 1/100
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Validations
Immunohistochemical analysis of paraffin-embedded human colon, using SOX9 Antibody .
Western blot analysis of extracts from SW480, HCT116 and 3T3 cells using Sox9 Rabbit pAb at 1:1000 dilution. Predicted band size: 70kDa. Observed band size: 70kDa.
Immunofluorescent analysis of SW480 cells, using SOX9 Antibody .
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Background
Swiss-Prot Acc.P48436.Regulates several important processes during embryonic development including chondrogenesis, during which it contributes to skeletal formation and digit specification (2,3). Sox9 also coordinates with steroidogenic factor-1 to direct Sertoli cell-specific expression of anti-Mullerian hormone during embryogenesis, thereby contributing to male sex determination.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"