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Product Name
Anti-Sox2 antibody
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Description
Rabbit polyclonal to Sox2
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Tested applications
WB, IHC-P, IP
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Species reactivity
Human Sox2
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Sox2, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 1-10 μg/mL
IHC-P: 0.1-2 μg/mL
IP: 0.5-2 μg/mg of lysate
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Validations
Sox2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Sox2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
Sox2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human Sox2 in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Sox2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human Sox2 in human glioma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (polypeptide).
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Background
SOX2, also known as ANOP3, is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. SOX2 is critical for early embryogenesis and for embryonic stem cell pluripotency. It keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Defects in SOX2 can cause microphthalmia, syndromic, 3. A disease characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
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References
- Lu Y, et al., 2010, PLoS ONE. 5(6): e11022.
- Gontan C, et al., 2008, Dev. Biol. 317 (1): 296-309.
- Shi W, et al., 2006, J. Biol. Chem. 281 (33): 23319-25.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"