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Product Name
Anti-SOX18 antibody
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Description
Rabbit monoclonal antibody to SOX18
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
HLTS antibody; HLTRS antibody
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Isotype
IgG
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Preparation
This antigen of this antibody was recombinant protein within human sox18 aa 250-384 / 384.
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Clonality
Monoclonal
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Formulation
Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:1,000
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Validations
Fig1:; Western blot analysis of SOX18 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
- Background
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References
- Petrovic, I. et al. SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines. PloS one 10: e0143591 (2015).
- Petrovic, I. et al. ZBP-89 and Sp3 down-regulate while NF-Y up-regulates SOX18 promoter activity in HeLa cells. Molecular biology reports 36: 993-1000 (2009).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"