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Product Name
Anti-Smad2 antibody
- Documents
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Description
Rabbit polyclonal to Smad2
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Tested applications
ELISA, WB, IHC-P, IF, ICC/IF
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Species reactivity
Mouse Smad2
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Alternative names
SMAD antibody; 7120426M23Rik antibody; hMAD-2 antibody; JV18 antibody; JV18-1 antibody; MAD homolog 2 antibody; MAD homolog 2 antibody; Madh2 antibody; MADH2 antibody; MADR2 antibody; Madr2 antibody; mad-related protein 2 antibody; mMad2 antibody; mother against DPP homolog 2 antibody; mothers against decapentaplegic homolog 2 antibody; mothers against decapentaplegic homolog 2 antibody; mothers against DPP homolog 2 antibody; mothers against DPP homolog 2 antibody; Sma- and Mad-related protein 2 antibody; Smad 2 antibody; SMAD family member 2 antibody; SMAD family member 2 antibody; Smad2 antibody; SMAD2 antibody; HSMAD2 antibody; JV18 antibody; MADH2 antibody; MADR2 antibody; JV18-1 antibody; hMAD-2 antibody; Madh2 antibody; Madr2 antibody; mMad2 antibody; Smad-2 antibody; 7120426M23Rik antibody; HSMAD2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse Smad2 (rM Smad2; Q62432; Ser2-Ser467). Smad2 specific IgG was purified by Mouse Smad2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Mouse Smad2.
IHC-P: 0.1-3 μg/mL
ICC/IF: 0.5-1.5 μg/mL
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Validations
Smad2 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of mouse SMAD2 in mouse heart with rabbit polyclonal antibody (2 µg/mL, formalin-fixed paraffin embedded sections).
Smad2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Smad2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of SMAD2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-mouse SMAD2 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.
Smad2 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of mouse SMAD2 in mouse skeletal muscle with rabbit polyclonal antibody (2 µg/mL, formalin-fixed paraffin embedded sections).
Smad2 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of mouse SMAD2 in mouse brain with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression.
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References
- Feng. et al., 2002, Mol Cell. 9 (1): 133-43.
- Zhu Y. et al., 1997, J Biol Chem. 272 (15): 10035-40.
- Zi Z. et al., 2012, FEBS Lett. 586 (14): 1921-8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"