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Anti-SET Rabbit antibody

Cat.#: 167043

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-SET Rabbit antibody
  • Documents
  • Description
    SET Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC, IP
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human SET
  • Clonality
    Polyclonal
  • Formulation
    Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at -20°C. Stable for 12 months from date of receipt.
  • Applications

    WB: 1/2000-1/10000

    IHC: 1/50

    ICC/IF: 1/200

    FC: 1/20

    IP: 1/20

  • Validations

    Western blot detection of SET in K562,Hela cell lysates using SET Rabbit pAb(1:1000 diluted).Predicted band size:34kDa.Observed band size:39kDa.

    Western blot detection of SET in K562,Hela cell lysates using SET Rabbit pAb(1:1000 diluted).Predicted band size:34kDa.Observed band size:39kDa.

    Immunocytochemistry of SET(green) in Hela cells using SET Rabbit pAb at dilution 1/50, and DAPI(blue)

    Immunocytochemistry of SET(green) in Hela cells using SET Rabbit pAb at dilution 1/50, and DAPI(blue)

  • Background
    Swiss-Prot Acc.Q01105.Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"