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Product Name
Anti-SERPINA7 antibody
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Description
Mouse monoclonal antibody to SERPINA7
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Tested applications
WB, ICC, IHC-P, FC
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Species reactivity
Human
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Alternative names
TBG antibody; TBGQTL antibody
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Isotype
IgG1
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Monoclonal
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Formulation
Liquid, 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:2,000
ICC: 1:100-1:200
IHC-P: 1:100-1:200
FC: 1:100-1:200
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Validations
Fig1: Western blot analysis of SERPINA7 on human SERPINA7 recombinant protein using anti-SERPINA7 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of SERPINA7 on HEK293 (1) and SERPINA7-hIgGFc transfected HEK293 (2) cell lysate using anti-SERPINA7 antibody at 1/1,000 dilution.
Fig3: ICC staining SERPINA7 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SERPINA7 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-SERPINA7 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of A431 cells with SERPINA7 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
- Background
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References
- Yan Z et al. Human thyroxine binding globulin (TBG) promoter directs efficient and sustaining transgene expression in liver-specific pattern. Gene 506(2):289-94 (2012).
- Duhan U et al. Explanation for the high heat stability of thyroxine binding globulin-Chicago. Endocr Regul 44(2):43-7 (2010).
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