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Product Name
Anti-S100A6 antibody
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Description
Rabbit monoclonal to S100A6
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse S100A6 / Calcyclin / CACY
No cross-reactivity in ELISA with Human S100A1; Human S100A2 / CAN19 / S100L -
Alternative names
2A9 antibody; 2A9 antibody; 5B10 antibody; 5B10 antibody; CABP antibody; CACY antibody; Cacy antibody; CALCYCLIN antibody; calcyclin antibody; PRA antibody; PRA antibody; S100a6 antibody; S100A6 antibody; 2A9 antibody; PRA antibody; 5B10 antibody; CABP antibody; CACY antibody; 2A9 antibody; PRA antibody; 5B10 antibody; Cacy antibody; CALCYCLIN antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse S100A6 / Calcyclin (rM S100A6; Met 1-Lys 89; NP_035443.1).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse S100A6. The detection limit for Mouse S100A6 is approximately 0.00245 ng/well.
IHC-P: 1-10 μg/mL
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Validations
S100A6 / Calcyclin / CACY Antibody, Rabbit MAb, Immunochemistry
Immunochemical staining of mouse S100A6 in mouse kidney with rabbit monoclonal antibody (5µg/mL, formalin-fixed paraffin embedded sections).
S100A6 / Calcyclin / CACY Antibody, Rabbit MAb, Immunochemistry
Immunochemical staining of mouse S100A6 in mouse spleen with rabbit monoclonal antibody (5µg/mL, formalin-fixed paraffin embedded sections).
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Background
S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. S100A6 (S100 calcium binding protein A6) is a member of the S100 family of proteins, and functions in prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of S100A6 have been implicated in melanoma.
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References
- Schäfer, B.W. et al., 1996, Trends Biochem. Sci. 21 (4): 134-140.
- Donato,R. et al., 2003, Microsc. Res. Tech. 60 (6): 540-551.
- Nowotny, M. et al., 2003, J. Biol. Chem. 278 (29): 26923-26928.
- Nonaka D, et al., 2008, J. Cutan. Pathol. 35 (11): 1014-1019.
- Marenholz, I. et al., 2004, Biochem. Biophys. Res. Commun. 322 (4): 1111-22.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"