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Product Name
Anti-S100A5 antibody
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Description
Mouse monoclonal to S100A5
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Tested applications
ELISA, IF, ICC/IF
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Species reactivity
Human S100A5
No cross-reactivity in ELISA with E.coli cell lysate -
Alternative names
S100A5 antibody; S100A5 antibody; S100D antibody; S100D9 antibody; S100D antibody; S100D9 antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human S100A5 (rh S100A5; B48219; Met 1-Lys 110). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human S100A5. The detection limit for Human S100A5 is approximately 0.039 ng/well.
ICC/IF: 10-25 μg/mL
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Validations
S100A5 Antibody, Mouse MAb, Immunofluorescence
Immunofluorescence staining of Human S100A5 in A431 or Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human S100A5 monoclonal antibody (15 µg/ml). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
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Background
S100 protein?is a family of low molecular weight protein found in vertebrates characterized by two?EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is?100%?soluble in ammonium sulfate?at neutral?pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the?neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response.?? Protein S100-A5, also known as Protein S-100D, S100 calcium-binding protein A5, S100A5 and S100D, is a member of the S100 family which contains two?EF-hand domains. S100A5 is also a novel member of the EF-hand superfamily of calcium-binding proteins that is poorly characterized at the protein level. It is expressed in very restricted regions of the adult brain. From birth onwards, S100A5 remained a neuronal-specific protein, only located in a subpopulation of neurons in the spiral ganglion.
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References
- Sch?fer,B.W. et al., 2000, J Biol Chem. 275 (39):30623-30.
- Gebhardt, C. et al., 2006, Biochem Pharmacol. 72 (11):1622-31.
- Nonaka, D. et al., 2008, J. Cutan. Pathol. 35 (11): 1014-9.
- Lim, SY. et al., 2008, J Immunol. 181 (8): 5627-36.
- Heibeck TH. et al., 2009, J. Proteome Res. 8:3852-61.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"