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Product Name
Anti-S100A13 antibody
- Documents
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Description
Mouse monoclonal to S100A13
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Tested applications
ELISA, IF, ICC/IF
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Species reactivity
Human S100A13
No cross-reactivity with E.coli cell lysate in ELISA -
Alternative names
OTTHUMP00000034802 antibody; OTTMUSP00000024894 antibody; protein S100-A13 antibody; protein S100-A13 antibody; S100 calcium-binding protein A13 antibody; S100 calcium-binding protein A13 antibody; S100A13 antibody; S100a13 antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human S100A13 (rh S100A13; NP_001019381.1; Met 1-Lys 98). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human S100A13. The detection limit for Human S100A13 is approximately 0.31 ng/well.
ICC/IF: 10-25 μg/mL
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Validations
S100A13 Antibody, Mouse MAb, Immunofluorescence
Immunofluorescence staining of Human S100A13 in A431 or Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human S100A13 monoclonal antibody (15 µg/ml). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope). Positive staining was localized to nuclear.
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Background
S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A13, also known as S100 calcium-binding protein A13, is a member of the S-100 family. It contains two EF-hand domains. S100A13 binds two calcium ions per subunit and one copper ion. Binding of one copper ion does not interfere with calcium binding. S100A13 is required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine. S100A13 plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway.
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References
- Mandinova A. et al., 2003, J Cell Sci. 116: 2687-96.
- Arnesano F. et al., 2005, Angew Chem Int Ed. 44: 6341-4.
- Viemann D. et al., 2005, Blood. 105: 2955-62.
- Nakatani Y. et al., 2005, Mediators Inflamm. 2005: 280-92.
- Bjoerk P. et al., 2009, PLoS Biol. 7: E97-E97.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"