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Product Name
Anti-S100A1 antibody
- Documents
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Description
Rabbit monoclonal to S100A1
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Tested applications
IHC-P
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Species reactivity
Mouse S100A1
No cross-reactivity in ELISA with Mouse S100B -
Alternative names
AI266795 antibody; S100 antibody; S100 antibody; S100a antibody; S100A antibody; S100a1 antibody; S100A1 antibody; S100-alpha antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse S100A1 (rM S100A1; P56565; Gly2-Ser94).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
IHC-P: 1-10 μg/mL
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Validations
S100A1 Antibody, Rabbit MAb, Immunochemistry
Immunochemical staining of mouse S100A1 in mouse heart with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
S100A1 Antibody, Rabbit MAb, Immunochemistry
Immunochemical staining of human mouse S100A1 in mouse bone with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to chondrocyte.
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Background
S100A1 is a Ca2+binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation.This protein has been shown to function in the processes including stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation.. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.
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References
- Remppis, A .et al., 1996, Biochim. Biophs. Acta. 1313: 253-257.
- Most, P. et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98: 13889-13894
- Okada, M. et al., 2004, J. Biol. Chem. 279: 4221-4233.
- Schafer, W.E. et al., 1995, Genomics. 25: 638-643.
- Landar, A. et al., 1996, Cell. Calcium. 20: 279-285.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"