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Product Name
Anti-RTR (2B11) Mouse antibody
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Description
RTR (2B11) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human NR6A1 (AA: 65-118) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/400
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NR6A1 mouse mAb with DAB staining.
Western blot detection of GCNF in Jurkat,MCF7 and Hela cell lysates using GCNF mouse mAb (1:3000 diluted).Predicted band size:54KDa.Observed band size:54KDa.
Immunofluorescence analysis of HepG2 cells using NR6A1 mouse mAb (green). Blue
Western blot analysis using NR6A1 mouse mAb against K562 (1), NTERA-2 (2), HEK293 (3), HUVE-12 (4), and HeLa (5) cell lysate.
Immunohistochemical analysis of paraffin-embedded cerebellum tissues using NR6A1 mouse mAb with DAB staining.
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-GCNF mouse mAb (dilution 1:200).
Flow cytometric analysis of K562 cells using NR6A1 mouse mAb (green) and negative control (red).
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Background
Swiss-Prot Acc.Q15406.This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. The gene expresses at least alternatively spliced transcript variants.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"