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Product Name
Anti-Rsk 2/MAPKAP Kinase 1b (5H5) Mouse antibody
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Description
Rsk 2/MAPKAP Kinase 1b (5H5) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human RSK2 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Flow cytometric analysis of HepG2 cells using RSK2 mouse mAb (green) and negative control (red).
Western blot detection of RSK2 in K562,3T3 and Hela cell lysates using RSK2 mouse mAb (1:500 diluted).Predicted band size:80KDa.Observed band size:90KDa.
Western blot analysis using RSK2 mouse mAb against Hela (1), MCF-7 (2), and HepG2 (3) cell lysate.
Immunofluorescence analysis of HepG2 cells using RSK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Background
Swiss-Prot Acc.P51812.This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"