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Product Name
Anti-RNase A / Ribonuclease A / RNASE1 antibody
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Description
Rabbit polyclonal to RNase A / Ribonuclease A / RNASE1
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Tested applications
IHC-P
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Species reactivity
Human RNase A / Ribonuclease A / RNASE1
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Alternative names
AI574248 antibody; HP-RNase antibody; pancreatic antibody; RIB1 antibody; Rib1 antibody; RIB-1 antibody; Rib-1 antibody; ribonuclease 1 antibody; ribonuclease 1 antibody; ribonuclease A antibody; ribonuclease pancreatic antibody; ribonuclease pancreatic antibody; RNase 1 antibody; RNase 1 antibody; RNase A antibody; RNase A antibody; RNase upI-1 antibody; RNS1 antibody; RAC1 antibody; RIB1 antibody; RNS1 antibody; Rib1 antibody; Rib-1 antibody; AI574248 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human RNase A / Ribonuclease A / RNASE1 (rh RNase A / Ribonuclease A / RNASE1; P07998; Met1-Thr156). RNase A / Ribonuclease A / RNASE1 specific IgG was purified by Human RNase A / Ribonuclease A / RNASE1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
IHC-P: 0.1-2 μg/ml
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Validations
RNase A / Ribonuclease A / RNASE1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
RNase A, also known as ribonuclease A and RNASE1, belongs to ribonuclease A superfamily. It is a pancreatic-type of secretory ribonuclease. RNase A is a basic protein and its many positive charges are consistent with its binding to RNA (a poly-anion). More generally, RNase A is unusually polar or, rather, unusually lacking in hydrophobic groups, especially aliphatic ones. As a endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
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References
- Tubert P, et al. (2011) Interactions crucial for three-dimensional domain swapping in the HP-RNase variant PM8. Biophys J. 101(2):459-67.
- Vinayagam A, et al. (2011) A directed protein interaction network for investigating intracellular signal transduction. Sci Signal. 4(189):rs8.
- Fischer S, et al. (2011) Expression and localisation of vascular ribonucleases in endothelial cells. Thromb Haemost. 105(2):345-55.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"