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Product Name
Anti-RNA polymerase II CTD repeat YSPTSPS Rabbit antibody
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Description
RNA polymerase II CTD repeat YSPTSPS Rabbit monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human RNA polymerase II CTD repeat YSPTSPS
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
IHC: 1/100
ICC/IF: 1/100
FC: 1/20
IP: 1/20
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Validations
Western blot detection of RNA polymerase II CTD repeat YSPTSPS in Hela,A549,MCF-7,U251,HL-60 cell lysates using RNA polymerase II CTD repeat YSPTSPS Rabbit mAb(1:1000 diluted).Predicted band size:192kDa.Observed band size:250kDa.
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Background
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"