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Anti-RhoA/B/C Rabbit antibody

Cat.#: 167153

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Product Information

  • Product Name
    Anti-RhoA/B/C Rabbit antibody
  • Documents
  • Description
    RhoA/B/C Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthesized peptide derived from human Rho A + B + C
  • Clonality
    Polyclonal
  • Formulation
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500-1/2000

    IHC: 1/500-1/1000

    ICC/IF: 1/100-1/500

    FC: 1/200

  • Validations

    Immunofluorescent analysis of Hela cells, using Rho A + B + C Antibody .

    Immunofluorescent analysis of Hela cells, using Rho A + B + C Antibody .

    Western blot analysis of Rho A + B + C expression in SHSY-5Y and Rat Brain cell lysates using Rho A + B + C antibody at 1/1000 dilution.Predicted band size:22KDa.Observed band size:22KDa.

    Western blot analysis of Rho A + B + C expression in SHSY-5Y and Rat Brain cell lysates using Rho A + B + C antibody at 1/1000 dilution.Predicted band size:22KDa.Observed band size:22KDa.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rho A + B + C Antibody .

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rho A + B + C Antibody .

    Western blot analysis of Rho A + B + C expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.

    Western blot analysis of Rho A + B + C expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.

  • Background
    Swiss-Prot Acc.P61586,P62745,P08134.RhoA , RhoB , RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"