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Product Name
Anti-Renin antibody
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Description
Rabbit monoclonal to Renin
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Tested applications
ELISA, WB, IHC-P, IF, ICC/IF
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Species reactivity
Human Renin / REN / AngiotensinogenaseHas 20 % cross activity in ELISA with Mouse REN1 / Renin-1
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Renin (rh Renin; NP_000528.1; Met 1-Arg 406).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human RENIN. The detection limit for Human RENIN is approximately 0.00245 ng/well.
IHC-P: 1-10 μg/mL
ICC/IF: 10-25 μg/mL
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Validations
Renin / REN / Angiotensinogenase Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human RENIN in human kidney with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
Renin / REN / Angiotensinogenase Antibody, Rabbit MAb, Western blot
Renin / REN / Angiotensinogenase Antibody, Rabbit MAb, Immunofluorescence
Immunofluorescence staining of Human Renin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Renin monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
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Background
Mouse Renin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of the peptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"