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Product Name
Anti-RBBP5 (1C9) Mouse antibody
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Description
RBBP5 (1C9) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
RBBP 5; RBBP-5; RBBP5; RBBP5_HUMAN; RBQ 3; RBQ3; Retinoblastoma binding protein 5; Retinoblastoma binding protein RBQ3; Retinoblastoma binding protein5; Retinoblastoma-binding protein 5; Retinoblastoma-binding protein RBQ-3; SWD1; SWD1 antibody; Set1c WD40 repeat antibody
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Isotype
Mouse IgG2a
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Preparation
Antigen: Purified recombinant human RBBP5 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/100
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Validations
Western blot detection of RBBP5 in C2C12,THP-1,PC12 and 3T3 cell lysates using RBBP5 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-RBBP5 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).
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Background
Swiss-Prot Acc.Q15291.As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
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