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Product Name
Anti-Rabbit IgG (5B2) Mouse antibody
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Description
Rabbit IgG (5B2) Mouse monoclonal antibody
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Tested applications
WB, ELISA
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Species reactivity
Rabbit
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Alternative names
Antibody; Ig gamma chain C region; Ig kappa-b4 chain C region; Ig kappa-b5 chain C region; Ig kappa-b9 chain C region; Ig lambda chain C region; K-BAS. antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Rabbit IgG
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/50000
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Validations
Immunoprecipitation analysis of CHO-K1 cell lysates transfected with HA-tagged NGAL using HA-Tag Rabbit pAb (390001,1:100 diluted) (lane 2). Lane 1 contains rabbit IgG IP control lysate. All lanes are probed with HA-Tag Rabbit pAb (390001,1:1000 diluted) as the primary antibody. Panel A incubated with secondary antibodies Anti-Rabbit whole IgG, HRP-linked Antibody for detecting.Panel B incubated with Anti-Rabbit IgG (Heavy-Chain Specific) mAb(1:50000 diluted) and the Anti-mouse whole IgG, HRP-linked Antibody was used for detecting.
Western blot detection of MEK-2 in THP-1,Jurkat,Raji,3T3 and Hela cell lysates using 310001(MEK-2 rabbit pAb,1:1000 diluted),then incubated with 168176(Rabbit IgG mAb,1:50000 diluted).Anti-mouse IgG, HRP-linked Antibody was used for detecting.Predicted band size:44KDa.Observed band size:44KDa.
Western blot analysis of decreasing concentrations of total rabbit IgG reduced and denatured in 2X SDS loading buffer with DTT using Mouse Anti-Rabbit IgG (Heavy-Chain Specific) mAb (1:1000 diluted).
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