Anti-PSME2 / PA28b antibody

Anti-PSME2 / PA28b antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

Mouse monoclonal to PSME2 / PA28b

ELISA, WB, IP

Human PSME2 / PA28b
No cross-reactivity in ELISA with E.coli cell lysate

proteasome (prosome antibody; macropain) activator subunit 2 (PA28 beta) antibody; PSME2 antibody; PA28B antibody; PA28beta antibody; REGbeta antibody; 11S regulator complex beta subunit antibody; 11S regulator complex subunit beta antibody; MCP activator antibody; 31-kD subunit antibody; REG-beta antibody; activator of multicatalytic protease subunit 2 antibody; cell migration-inducing protein 22 antibody; proteasome activator 28 subunit beta antibody; proteasome activator 28-beta antibody; proteasome activator complex subunit 2 antibody; proteasome activator hPA28 subunit beta antibody; PA28B antibody; REGbeta antibody; PA28beta antibody; PA28b antibody; AA589371 antibody; AI788882 antibody

Mouse IgG1

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PSME2 / PA28b (rh PSME2 / PA28b; PSME2_HUMAN; Met1-Tyr239). The IgG fraction of the cell culture supernatant was purified by Protein A affinity.

Monoclonal

0.2 μm filtered solution in PBS

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PSME2, also known as PA28b, is a subunit of proteasome. The 26S proteasome multicatalytic proteinase complex has a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME2 gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring.

• Ahn K. et al., 1996, J Biol Chem. 271 (30): 18237-42.
• Rual Jean-François. et al., 2005, Nature. 437 (7062): 1173-8.
• Ahn JY. et al., 1995, FEBS Lett. 366 (1): 37-42.