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Product Name
Anti-PSGL-1/CD162 antibody
- Documents
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Description
Rabbit polyclonal to PSGL-1/CD162
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse PSGL-1 / CD162
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Alternative names
CD162 antibody; CD162 antibody; CLA antibody; Psgl1 antibody; PSGL1 antibody; Psgl-1 antibody; PSGL-1 antibody; Selp1 antibody; Selpl antibody; SELPLG antibody; Selplg antibody; CLA antibody; CD162 antibody; PSGL1 antibody; PSGL-1 antibody; CD162 antibody; Psgl1 antibody; Selp1 antibody; Selpl antibody; Psgl-1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse PSGL-1 / CD162 (rh PSGL-1 / CD162; Q62170; Met1-Cys307). PSGL-1 / CD162 specific IgG was purified by Mouse PSGL-1 / CD162 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse PSGL-1 / CD162. The detection limit for Mouse PSGL-1 / CD162 is < 0.039 ng/well.
IHC-P: 0.1-1 μg/ml
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Validations
PSGL-1 / CD162 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~120 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"