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Product Name
Anti-Prohibitin (5H7) Mouse antibody
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Description
Prohibitin (5H7) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat, Monkey
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human PHB expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Western blot detection of Prohibitin in C6,COS7,3T3 and Hela cell lysates using Prohibitin mouse mAb (dilution 1:1000).Predicted band size:30 Kda.Observed band size:30KDa.
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Background
Swiss-Prot Acc.P35232.Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"