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Product Name
Anti-PRMT6 antibody
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Description
Rabbit polyclonal to PRMT6
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Tested applications
IF, ICC/IF
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Species reactivity
Human PRMT6
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Alternative names
HRMT1L6 antibody; Hrmt1l6 antibody; AW124876 antibody; BB233495 antibody; AW124876 antibody; BB233495 antibody; FLJ51477 antibody; Prmt6 antibody; FLJ10559 antibody; Hrmt1l6 antibody; HRMT1L6 antibody; PRMT6 antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PRMT6, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ICC/IF: 0.5-1.5 μg/mL
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Validations
PRMT6 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of PRMT6 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human PRMT6 polyclonal antibody (1 µg/ml)(Figure A), incubated with rabbit anti-human PRMT6 polyclonal antibody and antigen (Figure B) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.
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Background
Protein arginine N-methyltransferase 6, also known as Histone-arginine N-methyltransferase PRMT6, PRMT6, and HRMT1L6, is a member of the protein arginine N-methyltransferase family and PRMT6 subfamily. PRMT6 is highly expressed in kidney and testes. PRMT6 is known to catalyze the generation of asymmetric dimethylarginine in polypeptides. It has been implicated in human immunodeficiency virus pathogenesis, DNA repair, and transcriptional regulation. PRMT6 is known to methylate histone H3 Arg-2 (H3R2), and this negatively regulates the lysine methylation of H3K4 resulting in gene repression. PRMT6 plays a key role in coupling process by functioning as a transcriptional coactivator that can regulate alternative splicing. PRMT6 coactivates the progesterone, glucocorticoid and oestrogen receptors in luciferase reporter assays in a hormone-dependent manner. Small interfering RNA (siRNA) oligonucleotide duplex knockdown of PRMT6 disrupts oestrogen-stimulated transcription of endogenous GREB1 and progesterone receptor in MCF-7 breast cancer cells. Neutralizing the activity of PRMT6 could inhibit tumor progression and may be of cancer therapeutic significance.
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References
- Hyllus D, et al., 2007, Genes Dev. 21(24): 3369-80.
- Lakowski, TM. et al., 2008, J Biol Chem. 283 (15): 10015-25.
- Michaud-Levesque, J. et al., 2009, J Biol Chem. 284 (32): 21338-46.
- Harrison, MJ. et al., 2010, Nucleic acids Res. Jan 4.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"