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Product Name
Anti-PPP1A/PPP1CA (1H1) Mouse antibody
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Description
PPP1A/PPP1CA (1H1) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Alternative names
Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit; Catalytic subunit;EC 3.1.3.16;MGC15877;MGC1674;PP 1A;PP-1A;PP1A;PP1A_HUMAN;PP1alpha;PP2C ALPHA;PP2CA;Ppp1ca;Protein Phosphatase 2C Alpha Isoform;Serine threonine protein phos antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant human PPP1A protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/50
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Validations
Western blot detection of PPP1A in HepG2,MCF7 and Hela cell lysates using PPP1A mouse mAb (1:1000 diluted).Predicted band size:37KDa.Observed band size:37KDa.
Immunocytochemistry of HeLa cells using anti-PPP1A mouse mAb diluted 1:50.
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Background
Swiss-Prot Acc.P62136.Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity.
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