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Product Name
Anti-PPIL1 antibody
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Description
Rabbit polyclonal to PPIL1
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human PPIL1
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Alternative names
1110060O10Rik antibody; AI327391 antibody; CGI-124 antibody; cyclophilin-related gene 1 antibody; CYPL1 antibody; Cypl1 antibody; hCyPX antibody; peptidyl-prolyl cis-trans isomerase antibody; peptidyl-prolyl cis-trans isomerase-like 1 antibody; peptidyl-prolyl cis-trans isomerase-like 1 antibody; peptidylprolyl isomerase (cyclophilin)-like 1 antibody; peptidylprolyl isomerase (cyclophilin)-like 1 antibody; PPIase antibody; Ppil1 antibody; PPIL1 antibody; rotamase PPIL1 antibody; rotamase PPIL1 antibody; CYPL1 antibody; hCyPX antibody; CGI-124 antibody; Cypl1 antibody; AI327391 antibody; 1110060O10Rik antibody; PPIase antibody; PPIASE antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human PPIL1 (rh PPIL1; Q9Y3C6; Met1-Gly166). PPIL1 specific IgG was purified by Human PPIL1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human PPIL1. The detection limit for Human PPIL1 is < 0.039 ng/well.
IHC-P: 0.1-1 μg/ml
IP: 1-4 μg/mg of lysate
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Validations
PPIL1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
PPIL1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human PPIL1 in human epididymis with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
PPIL1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
PPIL1 is a member of the cyclophilin family. Cyclophilins are well conserved and ubiquitous. Members of cyclophilin family take an significant part in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. PPIL1 is a peptidylprolyl isomerase(PPIase). It increases the folding of proteins and catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIL1 is involved in proliferation of cancer cells through modulation of phosphorylation of stathmin. It is a novel molecular target for colon-cancer therapy.
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References
- Xu C. et al., 2005, J Biomol NMR. 31 (2): 179-80.
- Ozaki K. et al., 1997, Cytogenet Cell Genet. 72 (2-3): 242-5.
- Mann SS. et al., 1999, Cytogenet Cell Genet. 83 (3-4): 228-9.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"