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Product Name
Anti-Podoplanin antibody
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Description
Rabbit polyclonal to Podoplanin
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse PDPN / Podoplanin
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Alternative names
GP40 antibody; AGGRUS antibody; CTA-520D8.1 antibody; GP36 antibody; Gp38 antibody; Gp38 antibody; HT1A-1 antibody; OTS8 antibody; OTS-8 antibody; PA2.26 antibody; PA2.26 antibody; PDPN antibody; Pdpn antibody; Podoplanin antibody; Podoplanin antibody; RANDAM-2 antibody; RP23-348F1.2 antibody; T1A antibody; T1a antibody; T1A-2 antibody; T1alpha antibody; GP40 antibody; T1A antibody; GP36 antibody; Gp38 antibody; OTS8 antibody; T1A2 antibody; TI1A antibody; T1A-2 antibody; AGGRUS antibody; HT1A-1 antibody; PA2.26 antibody; T1a antibody; Gp38 antibody; OTS-8 antibody; T1alpha antibody; RANDAM-2 antibody; T1-alpha antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse PDPN / Podoplanin (rM PDPN / Podoplanin; NP_034459.2; Met1-Lys133). PDPN / Podoplanin specific IgG was purified by Mouse PDPN / Podoplanin affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse PDPN / Podoplanin. The detection limit for Mouse PDPN / Podoplanin is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
Podoplanin / PDPN Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse PDPN in mouse brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Podoplanin / PDPN Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse PDPN in mouse kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Podoplanin / PDPN Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse PDPN in mouse lung with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Podoplanin, also known as PDPN, is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined. Alternatively spliced transcript variants encoding different isoforms have been identified.PDPN is a mucin-type glycoprotein negatively charged by extensive O-glycosylation and a high content of sialic acid, which expresses the adhesive property. It is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. PDPN may contribute to form odontoblastic fiber or function as the anchorage to the tooth development and in proliferating epithelial cells of cervical loop and apical bud. The intensity of podoplanin expression is negatively correlated with the expression of CD34 and factor VIII. Podoplanin would be useful as a diagnostic marker for epithelioid hemangioendothelioma in liver tumors.
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References
- Kimura, N. et al., 2005, Pathol Int. 55 (2): 83-86.
- Ordóñez, N.G., 2006, Adv Anat Pathol. 13 (2): 83-88.
- Wicki, A. et al., 2007, Br. J. Cancer. 96 (1): 1-5.
- Fujii,T. et al., 2008, Mod Pathol. 21 (2): 125-130.
- Sawa,Y. et al., 2008, Acta Histochem Cytochem. 41 (5):121-126.
- Kaddu, S. et al., 2009, Am J Dermatopathol. 31 (2): 137-139.
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