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Product Name
Anti-PNLIP antibody
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Description
Rabbit monoclonal to PNLIP
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Tested applications
IHC-P
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Species reactivity
Human PNLIP
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Alternative names
PTL antibody; PNLIPD antibody; PTL antibody; 1810007A24Rik antibody; PL antibody; 1810007A24Rik antibody; pancreatic triacylglycerol lipase antibody; pancreatic triacylglycerol lipase antibody; pancreatic triglyceride lipase antibody; PL antibody; PNLIPD antibody; PTL antibody; PTL antibody; RP11-33D13.1 antibody; triacylglycerol acylhydrolase antibody; PL antibody; PL antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human PNLIP (rh PNLIP; P16233; Met1-Cys465).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
IHC-P: 0.5-5 μg/mL
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Validations
PNLIP Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human PNLIP in human pancreas with rabbit monoclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
PNLIP is an enzyme which belongs to the lipase family. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.
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References
- Hegele RA, et al. (2001) Polymorphisms in PNLIP, encoding pancreatic lipase, and associations with metabolic traits. J Hum Genet. 46(6):320-4.
- Thomas A, et al. (2005) Role of the lid hydrophobicity pattern in pancreatic lipase activity. J Biol Chem. 280(48):40074-83.
- Colin DY, et al. (2008) Exploring the active site cavity of human pancreatic lipase. Biochem Biophys Res Commun. 370(3):394-8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"