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Anti-Placental alkaline phosphatase (PLAP) Mouse antibody

Cat.#: 168311

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Product Information

  • Product Name
    Anti-Placental alkaline phosphatase (PLAP) Mouse antibody
  • Documents
  • Description
    Placental alkaline phosphatase (PLAP) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P
  • Species reactivity
    Human
  • Isotype
    Mouse IgG2b
  • Preparation
    Antigen: Purified recombinant fragment of human ALPP expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    ELISA: 1/10000

  • Validations

    Immunohistochemical analysis of paraffin-embedded ovarian cancer (A), stomach cancer (B) using ALPP mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded ovarian cancer (A), stomach cancer (B) using ALPP mouse mAb with DAB staining.

    Western blot detection of ALPP/PLAP in Hela,Human Embryo,HepG2 cell lysates using ALPP/PLAP (3C11) Mouse mAb(1:1000 diluted).Predicted band size:58KDa.Observed band size:70KDa.

    Western blot detection of ALPP/PLAP in Hela,Human Embryo,HepG2 cell lysates using ALPP/PLAP (3C11) Mouse mAb(1:1000 diluted).Predicted band size:58KDa.Observed band size:70KDa.

    Western blot analysis using ALPP mouse mAb against HepG2 (1), A431 (2) and MCF-7 (3) cell lysate.

    Western blot analysis using ALPP mouse mAb against HepG2 (1), A431 (2) and MCF-7 (3) cell lysate.

    Western blot analysis of extracts from HepG2 cell lysates using Placental alkaline phosphatase (PLAP) mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

    Western blot analysis of extracts from HepG2 cell lysates using Placental alkaline phosphatase (PLAP) mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

  • Background
    Swiss-Prot Acc.P05187.There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"