-
Product Name
Anti-PKN1 Rabbit antibody
- Documents
-
Description
PKN1 Rabbit polyclonal antibody
-
Tested applications
WB, FC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide of human PKN
-
Clonality
Polyclonal
-
Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
-
Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
-
Applications
WB: 1/1000-1/5000
FC: 1/20
-
Validations
Western blot detection of PKN in C6 cell lysates using PKN Rabbit pAb(1:1000 diluted).Predicted band size:104KDa.Observed band size:120KDa.
-
Background
Swiss-Prot Acc.Q16512.PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"