-
Product Name
Anti-PIM1 antibody
- Documents
-
Description
Rabbit polyclonal to PIM1
-
Tested applications
WB, IF, ICC/IF
-
Species reactivity
Human PIM1
-
Alternative names
PIM antibody; Pim-1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the human PIM1, and purified by antigen affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-30 μg/ml
ICC/IF: 0.5-1.5 μg/mL
-
Validations
PIM1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
PIM1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of PIM1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PIM1 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cytoplasm and nucleus.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"