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Product Name
Anti-Phospho-Rb2 p130 (Ser952) Rabbit antibody
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Description
Phospho-Rb2 p130 (Ser952) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse
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Alternative names
Rb2; P130 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser952 of human Rb2 p130
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000-1/10000
IHC: 1/20-1/100
ICC/IF: 1/20-1/100
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Validations
Western blot detection of Rb2 p130 (Phospho-Ser952) in K562,Hela cell lysates using Rb2 p130 (Phospho-Ser952) Rabbit pAb(1:1000 diluted).Predicted band size:128kDa.Observed band size:130kDa.
Immunocytochemistry of Rb2 p130 (Phospho-Ser952)(green) in Hela cells using Rb2 p130 (Phospho-Ser952) Rabbit pAb at dilution 1/50, and DAPI(blue)
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Background
Swiss-Prot Acc.Q08999.Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
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