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Product Name
Anti-Phospho-Raf1 (Ser259) Rabbit antibody
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Description
Phospho-Raf1 (Ser259) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat
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Alternative names
NS5; CRAF; Raf-1; c-Raf; CMD1NN antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser259 of human Raf1
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/20
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Validations
Western blot detection of Phospho-Raf1 (Ser259) in K562,C6,3T3,Hela cell lysates using Phospho-Raf1 (Ser259) Rabbit pAb(1:1000 diluted).Predicted band size:73kDa.Observed band size:73kDa.
Immunohistochemistry of Raf1 (Phospho-Ser259) in paraffin-embedded Human tonsil using Raf1 (Phospho-Ser259) Rabbit pAb at dilution 1/20
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Background
Swiss-Prot Acc.P04049.Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
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