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Product Name
Anti-Phospho-PTEN (Ser380/Thr382/383) Rabbit antibody
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Description
Phospho-PTEN (Ser380/Thr382/383) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of threonine 380/382/383 (R-Y-S(p)-D-T(p)-T(p)-D-S) derived from Human PTEN.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000ICC:1/100-1/200IHC:1/50-1/100
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Validations
Immunofluorescence staining of methanol-fixed MEF cells using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .
Immunofluorescence staining of methanol-fixed Hela cells using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .
Western blot detection of PTEN(Phospho-Ser380/Thr382/Thr383) in A549 cells untreated or treated with Alkaline phosphatase using PTEN(Phospho-Ser380/Thr382/Thr383) Rabbit pAb (dilution 1:1000, upper) or u03b2-Actin Mouse mAb (200068-8F10, lower).Predicted band size:54kDa.Observed band size:54kDa.
Western blot analysis of extracts from Hela and C6 cells, treated with EGF or PMA, using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .
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Background
Swiss-Prot Acc.P60484.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"