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Product Name
Anti-Phospho-LAT (Tyr200) Rabbit antibody
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Description
Phospho-LAT (Tyr200) Rabbit monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Alternative names
LAT1; pp36; IMD52 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Tyr200 of human LAT
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/2000
ICC/IF: 1/50
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Validations
Western blot detection of Phospho-LAT (Tyr200) in Jurkat cell lysates using Phospho-LAT (Tyr200) Rabbit mAb(1:500 diluted).Predicted band size:28kDa.Observed band size:36-38kDa.
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Background
Swiss-Prot Acc.O43561.Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"