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Product Name
Anti-Phospho-Cytokeratin 8 (Ser23) Rabbit antibody
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Description
Phospho-Cytokeratin 8 (Ser23) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human
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Alternative names
K8; KO; CK8; CK-8; CYK8; K2C8; CARD2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser23 of human Cytokeratin 8
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000-1/10000
IHC: 1/20
ICC/IF: 1/20
IP: 1/20
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Validations
Immunocytochemistry of Cytokeratin 8 (Phospho-Ser23)(green) in Hela cells using Cytokeratin 8 (Phospho-Ser23) Rabbit pAb at dilution 1/50, and DAPI(blue)
Western blot detection of Phospho-Cytokeratin 8 (Ser23) in K562,Hela cell lysates using Phospho-Cytokeratin 8 (Ser23) Rabbit pAb(1:1000 diluted).Predicted band size:54kDa.Observed band size:54kDa.
Immunohistochemistry of Cytokeratin 8 (Phospho-Ser23) in paraffin-embedded Human colon cancer tissue using Cytokeratin 8 (Phospho-Ser23) Rabbit pAb at dilution 1/20
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Background
Swiss-Prot Acc.P05787.Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"