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Product Name
Anti-Phospho-CD18 (Ser745) Rabbit antibody
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Description
Phospho-CD18 (Ser745) Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser745 of human CD18
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
ICC/IF: 1/100
FC: 1/200
IP: 1/20
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Validations
Western blot detection of CD18 (Phospho-Ser745) in K562,Rat Brain,C6,3T3 cell lysates using CD18 (Phospho-Ser745) Rabbit pAb(1:1000 diluted).Predicted band size:85kDa.Observed band size:85kDa.
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Background
Swiss-Prot Acc.P05107.Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992, PubMed:28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"